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NC State University / College of Veterinary Medicine

Multipex assay

A multiplex real-time PCR assay amplifying fecal mitochondrial DNA (mtDNA) was developed that facilitates identifying potential sources of fecal contamination in surface waters. Primer/Probe sets have been designed for the following species:


Dairy cow

Single Assay 
Canada goose

Please let us know if we can help with any fecal source tracking issues by completing the AECL mtDNA assay form.

Related publications:

Caldwell JM, Raley ME, Levine JF. 2007. Mitochondrial Multiplex Real-Time Pcr As A Source Tracking Method In Fecal-Contaminated Surface Waters. Environmental Science and Technology  10:3277-3283.

Caldwell, JM, Levine, JF. 2009. Domestic wastewater influent profiling using mitochondrial real-time PCR for source tracking animal contamination. Journal of Microbiological Methods: Epub:
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