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NC State University / College of Veterinary Medicine

Multipex assay

A multiplex real-time PCR assay amplifying fecal mitochondrial DNA (mtDNA) was developed that facilitates identifying potential sources of fecal contamination in surface waters. Primer/Probe sets have been designed for the following species:

Human
Swine
Bovine

Dairy cow

Single Assay 
Sheep
Goat
Horse
Dog
Cat
Canada goose


Please let us know if we can help with any fecal source tracking issues by completing the AECL mtDNA assay form.

Related publications:

Caldwell JM, Raley ME, Levine JF. 2007. Mitochondrial Multiplex Real-Time Pcr As A Source Tracking Method In Fecal-Contaminated Surface Waters. Environmental Science and Technology  10:3277-3283.

Caldwell, JM, Levine, JF. 2009. Domestic wastewater influent profiling using mitochondrial real-time PCR for source tracking animal contamination. Journal of Microbiological Methods: Epub:
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